Development and Application of Functionalized Protein Binders in Multicellular Organisms

Bieli, D. and Alborelli, I. and Harmansa, S. and Matsuda, S. and Caussinus, E. and Affolter, Markus. (2016) Development and Application of Functionalized Protein Binders in Multicellular Organisms. International Review of Cell and Molecular Biology, 325. pp. 181-213.

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Official URL: http://edoc.unibas.ch/42413/

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Protein-protein interactions are crucial to almost all biological processes. Studying such interactions in their native environment is critical but not easy to perform. Recently developed genetically encoded protein binders were shown to function inside living cells. These molecules offer a new, direct way to assess protein function, distribution and dynamics in vivo. A widely used protein binder scaffold are the so-called nanobodies, which are derived from the variable domain of camelid heavy-chain antibodies. Another commonly used scaffold, the DARPins, is based on Ankyrin repeats. In this review, we highlight how these binders can be functionalized in order to study proteins in vivo during the development of multicellular organisms. It is to be anticipated that many more applications for such synthetic protein binders will be developed in the near future.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell Biology (Affolter)
UniBasel Contributors:Affolter, Markus and Matsuda, Shinya and Bieli, Dimitri and Alborelli, Ilaria and Harmansa, Stefan and Caussinus, Emmanuel
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Academic Press
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:18 May 2020 11:58
Deposited On:22 Nov 2016 09:18

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