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Non-structural carbohydrates in woody plants compared among laboratories

Quentin, Audrey G. and Pinkard, Elizabeth A. and Ryan, Michael G. and Tissue, David T. and Baggett, L. Scott and Adams, Henry D. and Maillard, Pascale and Marchand, Jacqueline and Landhäusser, Simon M. and Lacointe, André and Gibon, Yves and Anderegg, William R. L. and Asao, Shinichi and Atkin, Owen K. and Bonhomme, Marc and Claye, Caroline and Chow, Pak S. and Clément-Vidal, Anne and Davies, Noel W. and Dickman, L. Turin and Dumbur, Rita and Ellsworth, David S. and Falk, Kristen and Galiano, Luc`ia and Grünzweig, José M. and Hartmann, Henrik and Hoch, Günter and Hood, Sharon and Jones, Joanna E. and Koike, Takayoshi and Kuhlmann, Iris and Lloret, Francisco and Maestro, Melchor and Mansfield, Shawn D. and Mart`inez-Vilalta, Jordi and Maucourt, Mickael and McDowell, Nathan G. and Moing, Annick and Muller, Bertrand and Nebauer, Sergio G. and Niinemets, Ulo and Palacio, Sara and Piper, Frida and Raveh, Eran and Richter, Andreas and Rolland, Gaëlle and Rosas, Teresa and Saint Joanis, Brigitte and Sala, Anna and Smith, Renee A. and Sterck, Frank and Stinziano, Joseph R. and Tobias, Mari and Unda, Faride and Watanabe, Makoto and Way, Danielle A. and Weerasinghe, Lasantha K. and Wild, Birgit and Wiley, Erin and Woodruff, David R.. (2015) Non-structural carbohydrates in woody plants compared among laboratories. Tree physiology, 35 (11). pp. 1146-1165.

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Official URL: http://edoc.unibas.ch/40452/

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Abstract

Non-structural carbohydrates (NSC) in plant tissue are frequently quantified to make inferences about plant responses to environmental conditions. Laboratories publishing estimates of NSC of woody plants use many different methods to evaluate NSC. We asked whether NSC estimates in the recent literature could be quantitatively compared among studies. We also asked whether any differences among laboratories were related to the extraction and quantification methods used to determine starch and sugar concentrations. These questions were addressed by sending sub-samples collected from five woody plant tissues, which varied in NSC content and chemical composition, to 29 laboratories. Each laboratory analyzed the samples with their laboratory-specific protocols, based on recent publications, to determine concentrations of soluble sugars, starch and their sum, total NSC. Laboratory estimates differed substantially for all samples. For example, estimates for Eucalyptus globulus leaves (EGL) varied from 23 to 116 (mean = 56) mg g(-1) for soluble sugars, 6-533 (mean = 94) mg g-1 for starch and 53-649 (mean = 153) mg g-1 for total NSC. Mixed model analysis of variance showed that much of the variability among laboratories was unrelated to the categories we used for extraction and quantification methods (method category R-2 = 0.05-0.12 for soluble sugars, 0.10-0.33 for starch and 0.01-0.09 for total NSC). For EGL, the difference between the highest and lowest least squares means for categories in the mixed model analysis was 33 mg g-1 for total NSC, compared with the range of laboratory estimates of 596 mg g-1. Laboratories were reasonably consistent in their ranks of estimates among tissues for starch (r = 0.41-0.91), but less so for total NSC (r = 0.45-0.84) and soluble sugars (r = 0.11-0.83). Our results show that NSC estimates for woody plant tissues cannot be compared among laboratories. The relative changes in NSC between treatments measured within a laboratory may be comparable within and between laboratories, especially for starch. To obtain comparable NSC estimates, we suggest that users can either adopt the reference method given in this publication, or report estimates for a portion of samples using the reference method, and report estimates for a standard reference material. Researchers interested in NSC estimates should work to identify and adopt standard methods.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Botanik > Physiological Plant Ecology (Kahmen)
UniBasel Contributors:Hoch, Günter
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Heron Publishing
ISSN:0829-318X
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:05 Jul 2016 08:22
Deposited On:05 Jul 2016 08:22

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