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Isolation and fast selective determinatino of nor-abhietanoid diterpenoids from Perovskia atriplicifolia roots using LC-ESIMS/MS with multiple reaction monitoring

Ślusarczyk, S. and Topolski, J. and Domaradzki, K. and Adams, M. and Hamburger, Matthias and Matkowski, M.. (2015) Isolation and fast selective determinatino of nor-abhietanoid diterpenoids from Perovskia atriplicifolia roots using LC-ESIMS/MS with multiple reaction monitoring. Natural Product Communications , 10 (7). pp. 1149-1152.

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Official URL: http://edoc.unibas.ch/40177/

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Abstract

In the first part of this study we extracted, isolated, and identified the main diterpenoid constituents from the roots of a Central Asian medicinal and ornamental plant--Perovskia atriplicifolia Benth. Eight major nor-abietanoid pigments were obtained using NP silica gel column chromatography and preparative RP-HPLC: cryptotanshinone, 1-hydroxycryptotanhinone, miltirone, 1-oxomiltirone, tanshinone IIa, 1,2 didehydrotanshinone IIa, 1,2 didehydromiltirone, the non-quinone diterpenoid - arucadiol, as well as rosmarinic acid as a main phenolic compound. Secondly, we used the obtained compounds for fast and selective determination of the main diterpenes present in P. atriplicifolia root extract. After extraction with n-hexane, the quantitative analysis was carried out by LC-MS/MS with a triple quadrupole (qQq) mass detector without any prior clean-up step. Identification of the diterpenes was confirmed by multiple reaction monitoring (MRM) using the most representative transitions from the precursor ions, while the most sensitive transitions were used for quantification in a 19-minute run. Most of the isolated and analyzed compounds had not been previously reported from this species. This easily cultivated plant is a promising source of several pharmacologically valuable abietanoid diterpenoids.
Faculties and Departments:05 Faculty of Science > Departement Pharmazeutische Wissenschaften > Pharmazie > Pharmazeutische Biologie (Hamburger)
UniBasel Contributors:Hamburger, Matthias
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Natural Product Incorporation
ISSN:1555-9475
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:28 Nov 2017 10:48
Deposited On:14 Jun 2016 07:14

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