edoc

Cbx2 targets PRC1 to constitutive heterochromatin in mouse zygotes in a parent-of-origin-dependent manner

Tardat, Mathieu and Albert, Mareike and Kunzmann, Rico and Liu, Zichuan and Kaustov, Lilia and Thierry, Raphael and Duan, Shili and Brykczynska, Urszula and Arrowsmith, Cheryl H. and Peters, Antoine H. F. M.. (2015) Cbx2 targets PRC1 to constitutive heterochromatin in mouse zygotes in a parent-of-origin-dependent manner. Molecular cell, 58 (1). pp. 157-171.

[img] PDF - Accepted Version
8Mb

Official URL: http://edoc.unibas.ch/40114/

Downloads: Statistics Overview

Abstract

Polycomb repressive complexes PRC1 and PRC2 regulate expression of genes involved in proliferation and development. In mouse early embryos, however, canonical PRC1 localizes to paternal pericentric heterochromatin (pat-PCH), where it represses transcription of major satellite repeats. In contrast, maternal PCH (mat-PCH) is enriched for H3 lysine 9 tri-methylation (H3K9me3) and Hp1β. How PRC1 is targeted to pat-PCH, yet excluded from mat-PCH, has remained elusive. Here, we identify a PRC1 targeting mechanism that relies on Cbx2 and Hp1β. Cbx2 directs catalytically active PRC1 to PCH via its chromodomain (CDCbx2) and neighboring AT-hook (ATCbx2) binding to H3K27me3 and AT-rich major satellites, respectively. CDCbx2 prevents ATCbx2 from interacting with DNA at PCH marked by H3K9me3 and Hp1β. Loss-of-function studies show that Hp1β and not H3K9me3 prevents PRC1 targeting to mat-PCH. Our findings indicate that CDCbx2 and ATCbx2 separated by a short linker function together to integrate H3K9me3/HP1 and H3K27me3 states.
Faculties and Departments:09 Associated Institutions > Friedrich Miescher Institut FMI > Epigenetics > Epigenetic control of mouse germ cell and early embryonic development (Peters)
UniBasel Contributors:Peters, Antoine
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Cell Press
ISSN:1097-2765
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:30 Jun 2016 11:00
Deposited On:01 Feb 2016 08:01

Repository Staff Only: item control page