Biophysics (communication arising) : is rhodopsin dimeric in native retinal rods?

Fotiadis, Dimitrios and Liang, Yan and Filipek, Slawomir and Saperstein, David A. and Engel, Andreas and Palczewski, Krzysztof. (2003) Biophysics (communication arising) : is rhodopsin dimeric in native retinal rods? Nature, 426 (6962). p. 31.

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Official URL: http://edoc.unibas.ch/dok/A5262394

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Individual biological molecules can be imaged under physiological conditions by atomic force microscopy. Our results from AFM, supported by electron microscopy, revealed distinct rows of rhodopsin dimers and paracrystalline arrays in native murine disc membranes. This supramolecular arrangement was also found for the light-activated form, opsin. We counted 30,000-55,000 rhodopsin molecules per square micrometre, a packing density comparable to that measured by optical methods in amphibian discs. From the lattice vectors describing the paracrystalline arrays, a maximum possible packing density of 62,900 rhodopsin molecules per square micrometre was calculated. The rhodopsin dimers seen in AFM topographs have cytosolic protrusions separated by 3.8 nm, providing an ideal docking platform for arrestin, which has two binding grooves that are separated by 3.8 nm as well.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Engel)
UniBasel Contributors:Engel, Andreas H
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:28 Jan 2019 10:35
Deposited On:22 Mar 2012 13:23

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