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PET Imaging of Stroke-Induced Neuroinflammation in Mice Using [F]PBR06

Lartey, F. M. and Ahn, G. O. and Shen, B. and Cord, K. T. and Smith, T. and Chua, J. Y. and Rosenblum, S. and Liu, H. and James, M. L. and Chernikova, S. and Lee, S. W. and Pisani, L. J. and Tirouvanziam, R. and Chen, J. W. and Palmer, T. D. and Chin, F. T. and Guzman, R. and Graves, E. E. and Loo, B. W. and Jr., . (2014) PET Imaging of Stroke-Induced Neuroinflammation in Mice Using [F]PBR06. Molecular imaging & biology, Vol. 16, H. 1. pp. 109-117.

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Official URL: http://edoc.unibas.ch/dok/A6338578

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Abstract

PURPOSE: The purpose of this study is to evaluate the 18 kDa translocator protein (TSPO) radioligand [18F]N-fluoroacetyl-N-(2,5-dimethoxybenzyl)-2-phenoxyaniline ([18F]PBR06) as a positron emission tomography (PET) imaging biomarker of stroke-induced neuroinflammation in a rodent model. PROCEDURES: Stroke was induced by transient middle cerebral artery occlusion in Balb/c mice. Dynamic PET/CT imaging with displacement and preblocking using PK111195 was performed 3 days later. PET data were correlated with immunohistochemistry (IHC) for the activated microglial markers TSPO and CD68 and with autoradiography. RESULTS: [18F]PBR06 accumulation peaked within the first 5 min postinjection, then decreased gradually, remaining significantly higher in infarct compared to noninfarct regions. Displacement or preblocking with PK11195 eliminated the difference in [18F]PBR06 uptake between infarct and noninfarct regions. Autoradiography and IHC correlated well spatially with uptake on PET. CONCLUSIONS: [18F]PBR06 PET specifically images TSPO in microglial neuroinflammation in a mouse model of stroke and shows promise for imaging and monitoring microglial activation/neuroinflammation in other disease models.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Brain Ischemia and Regeneration (Guzman)
UniBasel Contributors:Guzman, Raphael
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:1536-1632
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:05 Jun 2015 08:52
Deposited On:05 Jun 2015 08:52

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