Cell-Permeant and Photocleavable Chemical Inducer of Dimerization

Zimmermann, Mirjam and Cal, Ruben and Janett, Elia and Hoffmann, Viktor and Bochet, Christian G. and Constable, Edwin and Beaufils, Florent and Wymann, Matthias P.. (2014) Cell-Permeant and Photocleavable Chemical Inducer of Dimerization. Angewandte Chemie International Edition, 53 (18). pp. 4717-4720.

PDF - Published Version
Available under License CC BY-NC-ND (Attribution-NonCommercial-NoDerivatives).


Official URL: http://edoc.unibas.ch/dok/A6337435

Downloads: Statistics Overview


Chemical inducers of dimerization (CIDs) have been developed to orchestrate protein dimerization and translocation. Here we present a novel photocleavable HaloTag- and SNAP-tag-reactive CID (MeNV-HaXS) with excellent selectivity and intracellular reactivity. Excitation at 360 nm cleaves the methyl-6-nitroveratryl core of MeNV-HaXS. MeNV-HaXS covalently links HaloTag- and SNAP-tag fusion proteins, and enables targeting of selected membranes and intracellular organelles. MeNV-HaXS-mediated translocation has been validated for plasma membrane, late endosomes, lysosomes, Golgi, mitochondria, and the actin cytoskeleton. Photocleavage of MeNV-HaXS liberates target proteins and provides access to optical manipulation of protein relocation with high spatiotemporal and subcellular precision. MeNV-HaXS supports kinetic studies of protein dynamics and the manipulation of subcellular enzyme activities, which is exemplified for Golgi-targeted cargo and the assessment of nuclear import kinetics.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Division of Biochemistry and Genetics > Cancer- and Immunobiology (Wymann)
05 Faculty of Science > Departement Chemie > Former Organization Units Chemistry > Anorganische Chemie (Constable)
UniBasel Contributors:Wymann, Matthias P. and Constable, Edwin Charles
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
Related URLs:
Identification Number:
edoc DOI:
Last Modified:05 Sep 2023 16:06
Deposited On:06 Feb 2015 09:59

Repository Staff Only: item control page