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Cell-Permeant and Photocleavable Chemical Inducer of Dimerization

Zimmermann, Mirjam and Cal, Ruben and Janett, Elia and Hoffmann, Viktor and Bochet, Christian G. and Constable, Edwin and Beaufils, Florent and Wymann, Matthias P.. (2014) Cell-Permeant and Photocleavable Chemical Inducer of Dimerization. Angewandte Chemie International Edition, 53 (18). pp. 4717-4720.

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Official URL: http://edoc.unibas.ch/dok/A6337435

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Abstract

Chemical inducers of dimerization (CIDs) have been developed to orchestrate protein dimerization and translocation. Here we present a novel photocleavable HaloTag- and SNAP-tag-reactive CID (MeNV-HaXS) with excellent selectivity and intracellular reactivity. Excitation at 360 nm cleaves the methyl-6-nitroveratryl core of MeNV-HaXS. MeNV-HaXS covalently links HaloTag- and SNAP-tag fusion proteins, and enables targeting of selected membranes and intracellular organelles. MeNV-HaXS-mediated translocation has been validated for plasma membrane, late endosomes, lysosomes, Golgi, mitochondria, and the actin cytoskeleton. Photocleavage of MeNV-HaXS liberates target proteins and provides access to optical manipulation of protein relocation with high spatiotemporal and subcellular precision. MeNV-HaXS supports kinetic studies of protein dynamics and the manipulation of subcellular enzyme activities, which is exemplified for Golgi-targeted cargo and the assessment of nuclear import kinetics.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Division of Biochemistry and Genetics > Cancer- and Immunobiology (Wymann)
05 Faculty of Science > Departement Chemie > Chemie > Anorganische Chemie (Constable)
UniBasel Contributors:Wymann, Matthias P. and Constable, Edwin Charles
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Wiley
ISSN:1433-7851
e-ISSN:1521-3773
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:03 May 2017 13:35
Deposited On:06 Feb 2015 09:59

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