edoc

The ultrastructure of Chlorobaculum tepidum revealed by cryo-electron tomography

Kudryashev, Misha and Aktoudianaki, Aikaterini and Dedoglou, Dimitrios and Stahlberg, Henning and Tsiotis, Georgios. (2014) The ultrastructure of Chlorobaculum tepidum revealed by cryo-electron tomography. Biochimica et biophysica acta, Vol. 1837, H. 10. pp. 1635-1642.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A6329111

Downloads: Statistics Overview

Abstract

Chlorobaculum (Cba) tepidum is a green sulfur bacterium that oxidizes sulfide, elemental sulfur, and thiosulfate for photosynthetic growth. As other anoxygenic green photosynthetic bacteria, Cba tepidum synthesizes bacteriochlorophylls for the assembly of a large light-harvesting antenna structure, the chlorosome. Chlorosomes are sac-like structures that are connected to the reaction centers in the cytoplasmic membrane through the BChl α-containing Fenna-Matthews-Olson protein. Most components of the photosynthetic machinery are known on a biophysical level, however, the structural integration of light harvesting with charge separation is still not fully understood. Despite over two decades of research, gaps in our understanding of cellular architecture exist. Here we present an in-depth analysis of the cellular architecture of the thermophilic photosynthetic green sulfur bacterium of Cba tepidum by cryo-electron tomography. We examined whole hydrated cells grown under different electron donor conditions. Our results reveal the distribution of chlorosomes in 3D in an unperturbed cell, connecting elements between chlorosomes and the cytoplasmic membrane and the distribution of reaction centers in the cytoplasmic membrane.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Stahlberg)
UniBasel Contributors:Stahlberg, Henning
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:0006-3002
Note:Publication type according to Uni Basel Research Database: Journal article
Related URLs:
Identification Number:
Last Modified:06 Feb 2015 09:58
Deposited On:06 Feb 2015 09:58

Repository Staff Only: item control page