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Reasons for false-positive lipoarabinomannan ELISA results in a Tanzanian population

Kroidl, Inge and Clowes, Petra and Mwakyelu, Joshua and Maboko, Leonard and Kiangi, Abubakary and Rachow, Andrea and Reither, Klaus and Jung, Jutta and Nsojo, Anthony and Saathoff, Elmar and Hoelscher, Michael. (2014) Reasons for false-positive lipoarabinomannan ELISA results in a Tanzanian population. Scandinavian journal of infectious diseases, Vol. 46, H. 2. pp. 144-148.

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Official URL: http://edoc.unibas.ch/dok/A6233645

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Abstract

Abstract Lipoarabinomannan (LAM), a cell wall component of mycobacteria, can be detected in the urine of tuberculosis (TB) patients. Advantages of this diagnostic include the ease of sample collection and test methods. However, as with most new TB diagnostics, LAM tests have been evaluated in well-controlled laboratory settings and subsequently need assessment under real working conditions. Our experience showed that the diagnosis of TB using the detection of LAM in urine under field conditions is prone to false-positive results due to contamination. Dust and soil, but also stool, seemed to lead to increased OD values and thus false-positive results of the enzyme-linked immunosorbent assay (ELISA) for LAM; however, contamination with blood, as well as bacterial or fungal organisms, had no influence. The collection of urine for the detection of LAM should therefore follow strict collection criteria in order to avoid contamination.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology > Tuberculosis Research (Gagneux)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
UniBasel Contributors:Reither, Klaus
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Almqvist och Wiksell
ISSN:0036-5548
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:15 Aug 2014 07:16
Deposited On:15 Aug 2014 07:16

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