edoc

PolyTB : a genomic variation map for Mycobacterium tuberculosis

Coll, Francesc and Preston, Mark and Guerra-Assunção, José Afonso and Hill-Cawthorn, Grant and Harris, David and Perdigão, João and Viveiros, Miguel and Portugal, Isabel and Drobniewski, Francis and Gagneux, Sebastien and Glynn, Judith R. and Pain, Arnab and Parkhill, Julian and McNerney, Ruth and Martin, Nigel and Clark, Taane G.. (2014) PolyTB : a genomic variation map for Mycobacterium tuberculosis. Tuberculosis, Vol. 94, H. 3. pp. 346-354.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A6271934

Downloads: Statistics Overview

Abstract

Tuberculosis (TB) caused by Mycobacterium tuberculosis (Mtb) is the second major cause of death from an infectious disease worldwide. Recent advances in DNA sequencing are leading to the ability to generate whole genome information in clinical isolates of M. tuberculosis complex (MTBC). The identification of informative genetic variants such as phylogenetic markers and those associated with drug resistance or virulence will help barcode Mtb in the context of epidemiological, diagnostic and clinical studies. Mtb genomic datasets are increasingly available as raw sequences, which are potentially difficult and computer intensive to process, and compare across studies. Here we have processed the raw sequence data (<1500 isolates, eight studies) to compile a catalogue of SNPs (n = 74,039, 63% non-synonymous, 51.1% in more than one isolate, i.e. non-private), small indels (n = 4810) and larger structural variants (n = 800). We have developed the PolyTB web-based tool (http://pathogenseq.lshtm.ac.uk/polytb) to visualise the resulting variation and important meta-data (e.g. in silico inferred strain-types, location) within geographical map and phylogenetic views. This resource will allow researchers to identify polymorphisms within candidate genes of interest, as well as examine the genomic diversity and distribution of strains. PolyTB source code is freely available to researchers wishing to develop similar tools for their pathogen of interest.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology (MPI) > Tuberculosis Research (Gagneux)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
UniBasel Contributors:Gagneux, Sebastien
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:1472-9792
Note:Publication type according to Uni Basel Research Database: Journal article
Related URLs:
Identification Number:
Last Modified:18 Jul 2014 09:10
Deposited On:18 Jul 2014 09:10

Repository Staff Only: item control page