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Restriction of DNA in Yersinia enterocolitica detected by recipient ability for a derepressed R factor from _Escherichia coli_

Cornelis, G. and Colson, C.. (1975) Restriction of DNA in Yersinia enterocolitica detected by recipient ability for a derepressed R factor from _Escherichia coli_. Journal of General Microbiology, Vol. 87, H. 2. pp. 285-291.

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Official URL: http://edoc.unibas.ch/dok/A5259293

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Abstract

A derepressed R factor, RY2drd2, was transferred at a frequency of 5x10minus 3 between two strains of Yersinia enterocolitica mated on a membrane. Under the same conditions transfer of this R factor from Escherichia coli to Y. enterocolitica was observed at a frequency of only 7-7x10minus 6. This frequency was greatly increased when the recipient strain was heat-treated before mating. Heat exposure for optimum fertility was 50 to 52 degrees C for a period of 2 to 3 min. Mutants of Y. enterocolitica were isolated which were infected by RY2drd2 from E. coli or from Y. enterocolitica at the same frequency. These observations strongly suggest that a DNA restriction and modification system in Y. enterocolitica causes its low recipient ability for plasmids from other species.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Molecular Microbiology (Cornelis)
UniBasel Contributors:Cornelis, Guy R.
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Cambridge University Press
ISSN:0022-1287
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:21
Deposited On:22 Mar 2012 13:22

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