Michiels, T. and Popoff, M. Y. and Durviaux, S. and Coynault, C. and Cornelis, G.. (1987) A new method for the physical and genetic mapping of large plasmids : application to the localisation of the virulence determinants on the 90 kb plasmid of Salmonella typhimurium. Microbial pathogenesis, Vol. 3, H. 2. pp. 109-116.
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Official URL: http://edoc.unibas.ch/dok/A5259266
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Abstract
A new method based on transposon-promoted deletions was used to generate a set of deletions in the 90 kb virulence plasmid of Salmonella typhimurium. The analysis of 16 deletion mutants allowed: (1) construction of the restriction map of the plasmid for HindIII, BamHI and BglII; (2) localisation of the plasmid region involved in virulence; (3) identification of two functional replicons on the plasmid.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Molecular Microbiology (Cornelis) |
|---|---|
| UniBasel Contributors: | Cornelis, Guy R. |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | Academic Press |
| ISSN: | 0882-4010 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
| Last Modified: | 22 Mar 2012 14:20 |
| Deposited On: | 22 Mar 2012 13:22 |
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