Detection of pYV+ Yersinia enterocolitica isolates by P1 slide agglutination

Sory, M. P. and Tollenaere, J. and Laszlo, C. and Biot, T. and Cornelis, G. R. and Wauters, G.. (1990) Detection of pYV+ Yersinia enterocolitica isolates by P1 slide agglutination. Journal of clinical microbiology, Vol. 28, H. 11. pp. 2403-2408.

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Official URL: http://edoc.unibas.ch/dok/A5259252

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Rabbit polyclonal antisera were raised against the pYV-encoded outer membrane protein P1 of five Yersinia enterocolitica strains belonging to serogroups O:3, O:5,27, O:8, and O:9. Analysis of these strains with the sera showed that P1 presented at least six different antigenic factors. Two of the serum specimens were chosen to test the P1 agglutinability of 797 strains isolated from various sources. This technique appeared to be more reliable than autoagglutination and Ca2+ dependency to monitor the presence of the pYV plasmid. Hence, we propose this P1-mediated agglutination as a new and easy virulence test.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Molecular Microbiology (Cornelis)
UniBasel Contributors:Cornelis, Guy R.
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:20
Deposited On:22 Mar 2012 13:22

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