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Function and molecular architecture of the Yersinia injectisome tip complex

Broz, P. and Mueller, C. A. and Muller, S. A. and Philippsen, A. and Sorg, I. and Engel, A. and Cornelis, G. R.. (2007) Function and molecular architecture of the Yersinia injectisome tip complex. Molecular microbiology, Vol. 65, H. 5. pp. 1311-1320.

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Official URL: http://edoc.unibas.ch/dok/A5259134

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Abstract

By quantitative immunoblot analyses and scanning transmission electron microscopy (STEM), we determined that the needle of the Yersinia enterocolitica E40 injectisome consists of 139 +/- 19 YscF subunits and that the tip complex is formed by three to five LcrV monomers. A pentamer represented the best fit for an atomic model of this complex. The N-terminal globular domain of LcrV forms the base of the tip complex, while the central globular domain forms the head. Hybrids between LcrV and its orthologues PcrV (Pseudomonas aeruginosa) or AcrV (Aeromonas salmonicida) were engineered and recombinant Y. enterocolitica expressing the different hybrids were tested for their capacity to form the translocation pore by a haemolysis assay. There was a good correlation between haemolysis, insertion of YopB into erythrocyte membranes and interaction between YopB and the N-terminal globular domain of the tip complex subunit. Hence, the base of the tip complex appears to be critical for the functional insertion of YopB into the host cell membrane.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Engel)
05 Faculty of Science > Departement Biozentrum > Infection Biology
05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Molecular Microbiology (Cornelis)
UniBasel Contributors:Broz, Petr and Engel, Andreas H and Cornelis, Guy R. and Sorg, Isabel and Müller, Shirley
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Blackwell
ISSN:0950-382X
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:01 Feb 2013 08:44
Deposited On:22 Mar 2012 13:21

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