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Silencing of the SEC62 gene inhibits migratory and invasive potential of various tumor cells

Greiner, Markus and Kreutzer, Birgit and Jung, Volker and Grobholz, Rainer and Hasenfus, Andrea and Stöhr, Robert Franz and Tornillo, Luigi and Dudek, Johanna and Stöckle, Michael and Unteregger, Gerhard and Kamradt, Jörn and Wullich, Bernd and Zimmermann, Richard. (2011) Silencing of the SEC62 gene inhibits migratory and invasive potential of various tumor cells. International journal of cancer, Vol. 128, H. 10. pp. 2284-2295.

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Official URL: http://edoc.unibas.ch/dok/A6006994

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Abstract

Sec62 is part of the protein translocation apparatus in the membrane of the endoplasmic reticulum (ER). In yeast, Sec62 participates in the post-translational translocation of proteins into the ER, but its function in mammals remains elusive. Previously we described the amplification and over-expression of the SEC62 gene in prostate cancer cell lines and the protein has been described as a potential target gene in prostate cancer. In the current study we show that in the tumor tissue of prostate cancer patients Sec62 protein levels are elevated compared with tumor-free tissue derived from the same patients or from prostates of control group patients and that the higher Sec62 protein content correlates with an increasing de-differentiation of the cells. Therefore, up-regulation of Sec62 protein content indeed is a phenomenon associated with prostate cancer progression. Analysis of a multi-tissue tumor array showed that in addition to prostate cancer, overproduction of Sec62 is observed in various other tumors, most significantly in tumors of the lung and the thyroid. To examine the tumor-related functions of Sec62, we silenced the SEC62 gene in the prostate cancer cell-line PC3 as well as in a set of other tumor cell-lines with two different siRNAs. In general, after silencing of SEC62 the cell migration and the invasive potential of the cells was blocked or at least dramatically reduced while cell viability was hardly affected. Thus, the SEC62 gene may indeed be considered as a target gene in the therapy of various tumors.
Faculties and Departments:03 Faculty of Medicine > Bereich Querschnittsfächer (Klinik) > Pathologie USB
03 Faculty of Medicine > Departement Klinische Forschung > Bereich Querschnittsfächer (Klinik) > Pathologie USB
UniBasel Contributors:Tornillo, Luigi
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Alan R. Liss
ISSN:0020-7136
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:27 Mar 2014 13:13
Deposited On:27 Mar 2014 13:13

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