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Characterization by Mass Spectroscopy of a 10 kDa c-554 Cytochrome from the Green Sulfur Bacterium Chlorobium tepidum

Remigy, H. W. and Aivaliotis, M. and Ioannidis, N. and Jeno, P. and Mini, T. and Engel, A. and Jaquinod, M. and Tsiotis, G.. (2003) Characterization by Mass Spectroscopy of a 10 kDa c-554 Cytochrome from the Green Sulfur Bacterium Chlorobium tepidum. Photosynthesis research, Vol. 78, H. 2. pp. 153-160.

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Official URL: http://edoc.unibas.ch/dok/A6211760

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Abstract

Preparative isoelectric focusing was used to isolate a type c cytochrome from photosynthetic membranes of the green sulfur bacterium Chlorobium tepidum. The purified protein showed a molecular weight of 10 kDa according to SDS-PAGE and ESI mass spectrometry. The absorption spectrum in the visible range is typical of a cytochrome with peaks at 420, 525.2 and 554.4 nm. Cleavage by either trypsin or endoproteinase lys-C of the isolated cytochrome combined with tandem mass spectrometry and Edman sequencing yielded a sequence perfectly matching parts of the recently sequenced genome of C. tepidum.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Services Biozentrum > Mass Spectrometry (Jenö)
UniBasel Contributors:Jenö, Paul
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Kluwer
ISSN:0166-8595
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:31 Jan 2014 09:49
Deposited On:31 Jan 2014 09:49

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