Remigy, H. W. and Aivaliotis, M. and Ioannidis, N. and Jeno, P. and Mini, T. and Engel, A. and Jaquinod, M. and Tsiotis, G.. (2003) Characterization by Mass Spectroscopy of a 10 kDa c-554 Cytochrome from the Green Sulfur Bacterium Chlorobium tepidum. Photosynthesis research, Vol. 78, H. 2. pp. 153-160.
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Official URL: http://edoc.unibas.ch/dok/A6211760
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Abstract
Preparative isoelectric focusing was used to isolate a type c cytochrome from photosynthetic membranes of the green sulfur bacterium Chlorobium tepidum. The purified protein showed a molecular weight of 10 kDa according to SDS-PAGE and ESI mass spectrometry. The absorption spectrum in the visible range is typical of a cytochrome with peaks at 420, 525.2 and 554.4 nm. Cleavage by either trypsin or endoproteinase lys-C of the isolated cytochrome combined with tandem mass spectrometry and Edman sequencing yielded a sequence perfectly matching parts of the recently sequenced genome of C. tepidum.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Mass Spectrometry (Jenö) |
|---|---|
| UniBasel Contributors: | Jenö, Paul |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | Kluwer |
| ISSN: | 0166-8595 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
| Last Modified: | 31 Jan 2014 09:49 |
| Deposited On: | 31 Jan 2014 09:49 |
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