Molecular characterization of geminivirus-derived small RNAs in different plant species

Akbergenov, Rashid and Si-Ammour, Azeddine and Blevins, Todd and Amin, Imran and Kutter, Claudia and Vanderschuren, Herve and Zhang, Peng and Gruissem, Wilhelm and Meins, Frederick and Hohn, Thomas and Pooggin, Mikhail M.. (2006) Molecular characterization of geminivirus-derived small RNAs in different plant species. Nucleic Acids Research, 34 (2). pp. 462-471.

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Official URL: http://edoc.unibas.ch/dok/A6205229

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DNA geminiviruses are thought to be targets of RNA silencing. Here, we characterize small interfering (si) RNAs-the hallmarks of silencing-associated with Cabbage leaf curl begomovirus in Arabidopsis and African cassava mosaic begomovirus in Nicotiana benthamiana and cassava. We detected 21, 22 and 24 nt siRNAs of both polarities, derived from both the coding and the intergenic regions of these geminiviruses. Genetic evidence showed that all the 24 nt and a substantial fraction of the 22 nt viral siRNAs are generated by the dicer-like proteins DCL3 and DCL2, respectively. The viral siRNAs were 5' end phosphorylated, as shown by phosphatase treatments, and methylated at the 3'-nucleotide, as shown by HEN1 miRNA methylase-dependent resistance to beta-elimination. Similar modifications were found in all types of endogenous and transgene-derived siRNAs tested, but not in a major fraction of siRNAs from a cytoplasmic RNA tobamovirus. We conclude that several distinct silencing pathways are involved in DNA virus-plant interactions.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Ehemalige Einheiten Umweltwissenschaften > Molekulare Pflanzenvirologie (Hohn)
UniBasel Contributors:Pooggin, Mikhail
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Oxford University Press
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:10 Oct 2017 09:16
Deposited On:31 Jan 2014 09:49

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