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Junctate is a key element in calcium entry induced by activation of InsP3 receptors and/or calcium store depletion

Treves, Susan and Franzini-Armstrong, Clara and Moccagatta, Luca and Arnoult, Christophe and Grasso, Cristiano and Schrum, Adam and Ducreux, Sylvie and Zhu, Michael X. and Mikoshiba, Katsuhiko and Girard, Thierry and Smida-Rezgui, Sophia and Ronjat, Michel and Zorzato, Francesco. (2004) Junctate is a key element in calcium entry induced by activation of InsP3 receptors and/or calcium store depletion. Journal of Cell Biology, 166 (4). pp. 537-548.

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Official URL: http://edoc.unibas.ch/dok/A6174320

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Abstract

In many cell types agonist-receptor activation leads to a rapid and transient release of Ca(2+) from intracellular stores via activation of inositol 1,4,5 trisphosphate (InsP(3)) receptors (InsP(3)Rs). Stimulated cells activate store- or receptor-operated calcium channels localized in the plasma membrane, allowing entry of extracellular calcium into the cytoplasm, and thus replenishment of intracellular calcium stores. Calcium entry must be finely regulated in order to prevent an excessive intracellular calcium increase. Junctate, an integral calcium binding protein of endo(sarco)plasmic reticulum membrane, (a) induces and/or stabilizes peripheral couplings between the ER and the plasma membrane, and (b) forms a supramolecular complex with the InsP(3)R and the canonical transient receptor potential protein (TRPC) 3 calcium entry channel. The full-length protein modulates both agonist-induced and store depletion-induced calcium entry, whereas its NH(2) terminus affects receptor-activated calcium entry. RNA interference to deplete cells of endogenous junctate, knocked down both agonist-activated calcium release from intracellular stores and calcium entry via TRPC3. These results demonstrate that junctate is a new protein involved in calcium homeostasis in eukaryotic cells.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Perioperative Patient Safety (Girard/Treves)
UniBasel Contributors:Treves, Susan
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Rockefeller University Press
ISSN:0021-9525
e-ISSN:1540-8140
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:15 May 2017 08:57
Deposited On:25 Oct 2013 08:33

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