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Bioluminescence imaging to study the promoter activity of hla of Staphylococcus aureus in vitro and in vivo

Steinhuber, A. and Landmann, R. and Goerke, C. and Wolz, C. and Flückiger, U.. (2008) Bioluminescence imaging to study the promoter activity of hla of Staphylococcus aureus in vitro and in vivo. Zentralblatt für Bakteriologie, Vol. 298, H. 7/8. pp. 599-605.

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Official URL: http://edoc.unibas.ch/dok/A6006091

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Abstract

Alpha-toxin (Hla, encoded by hla) is a major virulence factor of Staphylococcus aureus. The activity of the hla promoter was analyzed using luxABCDE on an integration vector. The phla-lux construct was introduced in S. aureus Newman and its isogenic sae and sigB regulator mutants. Promoter activity was monitored by bioluminescence in vitro and in the murine tissue-cage model. Hla promoter activity could be followed in real time at repeated time points of infection. The activation of hla in the sigB-deficient strain and the repression to background levels in a sae-deficient strain relative to hla expression in the wild type could be demonstrated in vivo. Subinhibitory concentrations of teicoplanin, imipenem and ciprofloxacin enhanced hla promoter activity in vitro whereas clindamycin and rifampicin did not. Our approach proved to be rapid and adequate to study promoter activity in vitro and in vivo under conditions where high bacterial numbers are reached.
Faculties and Departments:03 Faculty of Medicine > Bereich Medizinische Fächer (Klinik) > Infektiologie > Infektiologie (Battegay M)
03 Faculty of Medicine > Departement Klinische Forschung > Bereich Medizinische Fächer (Klinik) > Infektiologie > Infektiologie (Battegay M)
03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Infection Biology (Khanna)
03 Faculty of Medicine > Departement Biomedizin > Former Units at DBM > Infection Biology (Landmann-Suter)
UniBasel Contributors:Flückiger, Ursula M. and Battegay, Manuel E. and Landmann-Suter, Regine
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:G. Fischer
ISSN:0934-8840
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:13 Sep 2013 08:00
Deposited On:13 Sep 2013 07:58

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