Pouched rats' detection of tuberculosis in human sputum : comparison to culturing and polymerase chain reaction

Mahoney, Amanda and Weetjens, Bart J. and Cox, Christophe and Beyene, Negussie and Reither, Klaus and Makingi, George and Jubitana, Maureen and Kazwala, Rudovick and Mfinanga, Godfrey S. and Kahwa, Amos and Durgin, Amy and Poling, Alan. (2012) Pouched rats' detection of tuberculosis in human sputum : comparison to culturing and polymerase chain reaction. Tuberculosis research and treatment, Vol. 2012 , 716989.

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Official URL: http://edoc.unibas.ch/dok/A6094319

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Setting. Tanzania. Objective. To compare microscopy as conducted in direct observation of treatment, short course centers to pouched rats as detectors of Mycobacterium tuberculosis. Design. Ten pouched rats were trained to detect tuberculosis in sputum using operant conditioning techniques. The rats evaluated 910 samples previously evaluated by smear microscopy. All samples were also evaluated through culturing and multiplex polymerase chain reaction was performed on culture growths to classify the bacteria. Results. The patientwise sensitivity of microscopy was 58.0%, and the patient-wise specificity was 97.3%. Used as a group of 10 with a cutoff (defined as the number of rat indications to classify a sample as positive for Mycobacterium tuberculosis) of 1, the rats increased new case detection by 46.8% relative to microscopy alone. The average samplewise sensitivity of the individual rats was 68.4% (range 61.1-73.8%), and the mean specificity was 87.3% (range 84.7-90.3%). Conclusion. These results suggest that pouched rats are a valuable adjunct to, and may be a viable substitute for, sputum smear microscopy as a tuberculosis diagnostic in resource-poor countries.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology (MPI) > Tuberculosis Ecology and Evolution Unit (Gagneux)
UniBasel Contributors:Reither, Klaus
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:04 Sep 2015 14:32
Deposited On:19 Jul 2013 07:42

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