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Using Drosophila melanogaster to validate metabolism-based insecticide resistance from insect pests

Daborn, P. J. and Lumb, C. and Harrop, T. W. and Blasetti, A. and Pasricha, S. and Morin, S. and Mitchell, S. N. and Donnelly, M. J. and Müller, P. and Batterham, P.. (2012) Using Drosophila melanogaster to validate metabolism-based insecticide resistance from insect pests. Insect biochemistry and molecular biology, Vol. 42, H. 12. pp. 918-924.

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Official URL: http://edoc.unibas.ch/dok/A6094442

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Abstract

Identifying molecular mechanisms of insecticide resistance is important for preserving insecticide efficacy, developing new insecticides and implementing insect control. The metabolic detoxification of insecticides is a widespread resistance mechanism. Enzymes with the potential to detoxify insecticides are commonly encoded by members of the large cytochrome P450, glutathione S-transferase and carboxylesterase gene families, all rapidly evolving in insects. Here, we demonstrate that the model insect Drosophila melanogaster is useful for functionally validating the role of metabolic enzymes in conferring metabolism-based insecticide resistance. Alleles of three well-characterized genes from different pest insects were expressed in transgenic D. melanogaster : a carboxylesterase gene (alphaE7) from the Australian sheep blowfly Lucilia cuprina, a glutathione S-transferase gene (GstE2) from the mosquito Anopheles gambiae and a cytochrome P450 gene (Cyp6cm1) from the whitefly Bemisia tabaci. For all genes, expression in D. melanogaster resulted in insecticide resistance phenotypes mirroring those observed in resistant populations of the pest species. Using D. melanogaster to assess the potential for novel metabolic resistance mechanisms to evolve in pest species is discussed
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Health Interventions > Vector Control Centre (Müller)
UniBasel Contributors:Müller, Pie
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Pergamon Press
ISSN:0965-1748
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:16 Feb 2018 13:59
Deposited On:19 Jul 2013 07:42

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