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Noninvasive genotyping fetal Kell blood group (KEL1) using cell-free fetal DNA in maternal plasma by MALDI-TOF mass spectrometry

Li, Ying and Finning, Kirstin and Daniels, Geoff and Hahn, Sinuhe and Zhong, Xiaoyan and Holzgreve, Wolfgang. (2008) Noninvasive genotyping fetal Kell blood group (KEL1) using cell-free fetal DNA in maternal plasma by MALDI-TOF mass spectrometry. Prenatal diagnosis, Vol. 28, H. 3. pp. 203-208.

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Official URL: http://edoc.unibas.ch/dok/A6003929

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Abstract

BACKGROUND: Alloimmunization against the fetal Kell (KEL1) blood group antigen is gaining importance relative to the Rhesus problem and is the second most important cause of hemolytic disease of the fetus and newborn. Molecular diagnosis for fetal KEL1, which currently involves invasive procedures, is routinely done for accessing whether a fetus is at risk. Here we developed a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based single allele-based extension reaction (SABER) to examine the fetal KEL1 gene from KEL1-negative pregnant women using cell-free fetal DNA in maternal plasma. METHODS: Thirty-two maternal plasma samples taken at the second and third trimesters of gestation (median: 21.5 weeks) were examined with MALDI-TOF MS-based SABER. The results were confirmed by serological tests on cord blood or polymerase chain reaction (PCR) typing on amniocyte-derived fetal DNA. RESULTS: We were able to detect the fetal KEL1 allele in 11 of the 13 KEL1-positive samples. No false positive results were scored. The paternal KEL1 allele could be correctly determined in 94% of cases (30/32). CONCLUSIONS: Our results indicated that the MALDI-TOF MS-based SABER has been used successfully for the detection of the fetal KEL1 status with the accuracy of 94%. Further, large-scale study, such as multicenter study, can now be explored for clinical application.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Prenatal Medicine (Hahn)
03 Faculty of Medicine > Departement Biomedizin > Former Units at DBM > Gynecological Oncology (Zhong)
UniBasel Contributors:Zhong, Xiao Yan and Hahn, Sinuhe
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Wiley
ISSN:0197-3851
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:19 Jul 2013 07:44
Deposited On:19 Jul 2013 07:41

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