Stoeckli, E. T. and Lemkin, P. F. and Kuhn, T. B. and Ruegg, M. A. and Heller, M. and Sonderegger, P.. (1989) Identification of proteins secreted from axons of embryonic dorsal-root-ganglia neurons. European Journal of Biochemistry, 180 (2). pp. 249-258.
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Abstract
Secretion of proteins from the growth cone has been implicated in axon growth and synapse formation and might be involved in the transmission of a variety of axon-derived regulatory signals during neurogenesis. In order to identify axonally secreted proteins, dorsal-root-ganglia neurons from chicken embryos were cultured in a compartmentalized cell culture system that allows separate access to neuronal cell somas and axons. The proteins synthesized by the neurons were metabolically labeled by addition of [35S]methionine to the compartment containing the cell somas; the proteins released from the axons were harvested from the culture medium of the axonal compartment. Two-dimensional gel electrophoresis revealed two axonally secreted proteins with apparent molecular mass of 132-140 kDa and 54-60 kDa; they were termed axonin-1 and axonin-2, respectively. Both axonins were found to be secreted from a variety of neuronal cell cultures, but not from any of the nonneuronal cultures investigated, and hence might be neuron-specific. Virtual absence of these proteins from the axonal protein pattern suggests constitutive secretion. The information acquired on coordinates and spot morphology of these proteins in two-dimensional gel electrophoresis provides a useful assay for their purification.
Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Neurobiology > Pharmacology/Neurobiology (Rüegg) |
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UniBasel Contributors: | Rüegg, Markus A. |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | Blackwell |
ISSN: | 0014-2956 |
e-ISSN: | 1432-1033 |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Language: | English |
Identification Number: |
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edoc DOI: | |
Last Modified: | 17 Apr 2019 12:49 |
Deposited On: | 22 Mar 2012 13:20 |
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