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FRET imaging of cells transfected with siRNA/liposome complexes

Kim, Il-Han and Järve, Anne and Hirsch, Markus and Fischer, Roger and Trendelenburg, Michael F. and Massing, Ulrich and Rohr, Karl and Helm, Mark. (2010) FRET imaging of cells transfected with siRNA/liposome complexes. Methods in Molecular Biology, Vol. 606. pp. 439-455.

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Official URL: http://edoc.unibas.ch/dok/A6003307

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Abstract

By monitoring the efficiency of fluorescence resonance energy transfer of dyes attached to the different strands of siRNA, the structural integrity of the latter can be traced inside cells. Here, the experimental details of dye-labeled siRNA construction, tissue culture, and transfection with liposomally formulated siRNAs are given, as well as the conditions for confocal microscopy and an algorithm allowing the visualization of intact siRNA after image data treatment. The method allows rapid screening of different liposomal siRNA formulations, obtained by small scale dual asymmetric centrifugation with high entrapping efficiency.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Clinical Immunology (Trendelenburg)
UniBasel Contributors:Trendelenburg, Marten
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Humana Press
ISSN:1064-3745
e-ISSN:1940-6029
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:12 Oct 2017 10:06
Deposited On:21 Jun 2013 12:25

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