An anti-contamination cocktail for the in vitro isolation and cultivation of parasitic protozoa

Mäser, Pascal and Grether-Bühler, Yvonne and Kaminsky, Ronald and Brun, Reto. (2002) An anti-contamination cocktail for the in vitro isolation and cultivation of parasitic protozoa. Parasitology research : Organ der Deutschen Gesellschaft für Parasitologie, Vol. 88, H. 2. pp. 172-174.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A6124430

Downloads: Statistics Overview


Contamination by bacteria or fungi is a frequent problem with the in vitro isolation and propagation of protozoan parasites. We developed an antibiotic combination of broad anti-contaminant activity but minimal toxicity to protozoa. This anti-contamination cocktail consists of penicillin G (60 microgml(-1)), kanamycin (100 microgml(-1)), chloramphenicol (10 microgml(-1)) and flucytosine (50 microgml(-1)). It is well tolerated by African and South American trypanosomes, Leishmania spp., Giardia duodenalis, Entamoeba histolytica and Plasmodium falciparum, and it provides an effective means for the treatment of contaminated cultures. The anti-contamination cocktail meets numerous prophylactic applications in which sterile handling is impracticable or impossible. In particular, it facilitates the direct in vitro isolation of parasites from an infected host.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology (MPI) > Parasite Chemotherapy (Mäser)
UniBasel Contributors:Mäser, Pascal and Brun, Reto
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
Related URLs:
Identification Number:
Last Modified:24 May 2013 09:23
Deposited On:24 May 2013 09:19

Repository Staff Only: item control page