edoc

Ectosomes released by polymorphonuclear neutrophils induce a MerTK-dependent anti-inflammatory pathway in macrophages

Eken, Ceylan and Martin, Perrine J. and Sadallah, Salima and Treves, Susan and Schaller, Monica and Schifferli, Jürg A.. (2010) Ectosomes released by polymorphonuclear neutrophils induce a MerTK-dependent anti-inflammatory pathway in macrophages. Journal of Biological Chemistry, 285 (51). pp. 39914-39921.

[img]
Preview
PDF - Published Version
1476Kb

Official URL: http://edoc.unibas.ch/dok/A6003785

Downloads: Statistics Overview

Abstract

At the earliest stage of activation, human polymorphonuclear neutrophils release vesicles derived directly from the cell surface. These vesicles, called ectosomes (PMN-Ect), expose phosphatidylserine in the outer membrane leaflet. They inhibit the inflammatory response of human monocyte-derived macrophages and dendritic cells to zymosan A (ZymA) and LPS and induce TGF- 1 release, suggesting a reprogramming toward a tolerogenic phenotype. The receptors and signaling pathways involved have not yet been defined. Here, we demonstrate that PMN-Ect interfered with ZymA activation of macrophages via inhibition of NF B p65 phosphorylation and NF B translocation. The MerTK (Mer receptor tyrosine kinase) and PI3K/Akt pathways played a key role in this immunomodulatory effect as shown using specific MerTK-blocking antibodies and PI3K inhibitors LY294002 and wortmannin. As a result, PMN-Ect reduced the transcription of many proinflammatory genes in ZymA-activated macrophages. In sum, PMN-Ect interacted with the macrophages by activation of the MerTK pathway responsible for down-modulation of the proinflammatory signals generated by ZymA.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Former Units at DBM > Immunonephrology (Schifferli)
UniBasel Contributors:Schifferli, Jürg A.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Society of Biological Chemists
ISSN:0021-9258
e-ISSN:1083-351X
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:11 May 2017 14:08
Deposited On:24 May 2013 09:18

Repository Staff Only: item control page