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Use of isothermal microcalorimetry to monitor microbial activities

Braissant, Olivier and Wirz, Dieter and Göpfert, Beat and Daniels, Alma U.. (2010) Use of isothermal microcalorimetry to monitor microbial activities. FEMS Microbiology Letters, 303 (1). pp. 1-8.

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Official URL: http://edoc.unibas.ch/dok/A6007657

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Abstract

Isothermal calorimetry measures the heat flow of biological processes, which is proportional to the rate at which a given chemical or physical process takes place. Modern isothermal microcalorimeters make measurements of less than a microwatt of heat flow possible. As a result, as few as 10 000-100 000 active bacterial cells in culture are sufficient to produce a real-time signal dynamically related to the number of cells present and their activity. Specimens containing bacteria need little preparation, and isothermal microcalorimetry (IMC) is a nondestructive method. After IMC measurements, the undisturbed samples can be evaluated by any other means desired. In this review, we present a basic description of microcalorimetry and examples of microbiological applications of IMC for medical and environmental microbiology. In both fields, IMC has been used to quantify microbial activity over periods of hours or even days. Finally, the recent development of highly parallel instruments (up to 48 channels) and the constantly decreasing costs of equipment have made IMC increasingly attractive for microbiology. Miniaturization of isothermal calorimeters provides an even wider range of possibilities.
Faculties and Departments:03 Faculty of Medicine > Bereich Medizinische Fächer (Klinik) > Rheumatologie
03 Faculty of Medicine > Departement Klinische Forschung > Bereich Medizinische Fächer (Klinik) > Rheumatologie
UniBasel Contributors:Daniels, A.U. Dan and Göpfert, Beat
Item Type:Article, refereed
Article Subtype:Further Journal Contribution
Bibsysno:Link to catalogue
Publisher:Oxford University Press
ISSN:0378-1097
e-ISSN:1574-6968
Note:Publication type according to Uni Basel Research Database: Journal item
Identification Number:
Last Modified:29 Nov 2017 09:59
Deposited On:24 May 2013 09:14

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