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The raft-associated protein MAL is required for maintenance of proper axon--glia interactions in the central nervous system

Schaeren-Wiemers, Nicole and Bonnet, Annick and Erb, Michael and Erne, Beat and Bartsch, Udo and Kern, Frances and Mantei, Ned and Sherman, Diane and Suter, Ueli. (2004) The raft-associated protein MAL is required for maintenance of proper axon--glia interactions in the central nervous system. The Journal of cell biology, Vol. 166, H. 5. pp. 731-742.

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Official URL: http://edoc.unibas.ch/dok/A5838864

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Abstract

The myelin and lymphocyte protein (MAL) is a tetraspan raft-associated proteolipid predominantly expressed by oligodendrocytes and Schwann cells. We show that genetic ablation of mal resulted in cytoplasmic inclusions within compact myelin, paranodal loops that are everted away from the axon, and disorganized transverse bands at the paranode--axon interface in the adult central nervous system. These structural changes were accompanied by a marked reduction of contactin-associated protein/paranodin, neurofascin 155 (NF155), and the potassium channel Kv1.2, whereas nodal clusters of sodium channels were unaltered. Initial formation of paranodal regions appeared normal, but abnormalities became detectable when MAL started to be expressed. Biochemical analysis revealed reduced myelin-associated glycoprotein, myelin basic protein, and NF155 protein levels in myelin and myelin-derived rafts. Our results demonstrate a critical role for MAL in the maintenance of central nervous system paranodes, likely by controlling the trafficking and/or sorting of NF155 and other membrane components in oligodendrocytes.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Neurobiology (Schaeren-Wiemers)
UniBasel Contributors:Schaeren-Wiemers, Nicole
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
ISSN:0021-9525
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:26 Apr 2013 07:02
Deposited On:26 Apr 2013 06:57

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