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Sequence requirements in different steps of the pre-mRNA splicing reaction : analysis by the RNA modification-exclusion technique

Lang, K. M. and Keller, W.. (1990) Sequence requirements in different steps of the pre-mRNA splicing reaction : analysis by the RNA modification-exclusion technique. Molecular and Cellular Biology, 10 (9). pp. 4942-4947.

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Official URL: http://edoc.unibas.ch/dok/A5257993

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Abstract

The stepwise assembly of splicing complexes and the subsequent splicing reaction were analyzed by the RNA modification-exclusion technique, which generates the equivalent of a complete set of point mutations in a single reaction. We found that although the sequences surrounding the 5' splice site, the branch point, and the 3' splice site, including the 3' AG, were required for presplicing complex formation, modified nucleotides at these positions were not completely excluded. The same sequences were required for splicing complex formation; however, modified nucleotides in these sequences were excluded to a much greater extent.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Keller)
UniBasel Contributors:Keller, Walter
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:American Society for Microbiology
ISSN:0270-7306
e-ISSN:1098-5549
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:07 Nov 2017 14:00
Deposited On:22 Mar 2012 13:19

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