Martin, G. and Keller, W.. (1998) Tailing and 3'-end labeling of RNA with yeast poly(A) polymerase and various nucleotides. RNA, 4 (2). pp. 226-230.
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Abstract
We have tested conditions for the labeling and tailing the 3'-end of RNAs with yeast poly(A) polymerase. Conditions were optimized for addition of NTP, dNTP, or ddNTP nucleotides to RNA. ATP, GTP, and UTP were useful for adding homopolymer tracts of various lengths. The nonradioactive nucleotides biotin-N6-ATP and digoxigenin-11-UTP also were used efficiently.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Keller) |
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| UniBasel Contributors: | Keller, Walter |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | Cambridge University Press |
| ISSN: | 1355-8382 |
| e-ISSN: | 1469-9001 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
| Language: | English |
| Related URLs: | |
| Identification Number: |
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| edoc DOI: | |
| Last Modified: | 07 Nov 2017 16:19 |
| Deposited On: | 22 Mar 2012 13:19 |
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