Hierarchical self-assembly of actin in micro-confinements using microfluidics

Deshpande, Siddharth and Pfohl, Thomas. (2012) Hierarchical self-assembly of actin in micro-confinements using microfluidics. Biomicrofluidics, 6 . 034120.

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Official URL: http://edoc.unibas.ch/dok/A6070568

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We present a straightforward microfluidics system to achieve step-by-step reaction sequences in a diffusion-controlled manner in quasi two-dimensional micro-confinements. We demonstrate the hierarchical self-organization of actin (actin monomers—entangled networks of filaments—networks of bundles) in a reversible fashion by tuning the Mg2+ ion concentration in the system. We show that actin can form networks of bundles in the presence of Mg2+ without any cross-linking proteins. The properties of these networks are influenced by the confinement geometry. In square microchambers we predominantly find rectangular networks, whereas triangular meshes are predominantly found in circular chambers.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Former Organization Units Chemistry > Biophysikalische Chemie (Pfohl)
UniBasel Contributors:Pfohl, Thomas and Deshpande, Siddharth Rajendra
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:AIP Publishing
Note:Publication type according to Uni Basel Research Database: Journal article
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edoc DOI:
Last Modified:16 Feb 2017 09:53
Deposited On:01 Feb 2013 08:45

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