edoc

A micromethod for separation and identification of digestive enzymes in brush border membrane fragments of single human intestinal biopsies

Freiburghaus, A. U. and Hauri, H. P. and Green, J. and Hadorn, B.. (1978) A micromethod for separation and identification of digestive enzymes in brush border membrane fragments of single human intestinal biopsies. Clinica Chimica Acta, Vol. 86, H. 3. pp. 227-234.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A5257852

Downloads: Statistics Overview

Abstract

A micromethod for the isolation of brush border membrane fragments from single peroral duodenal biopsies, and their subsequent analysis by polyacrylamide gel electrophoresis is described. The quantity of biopsy material used varied between 5 and 15 mg wet weight, leaving enough mucosa for histological examination. By cutting the gels longitudinally into two halves it was possible to identify several maltases, sucrase, isomaltase and lactase and to correlate these enzymatic activities with distinct co-migrating protein peaks. For alkaline phosphatase and enterokinase this correlation was not possible. This method is suitable for the study on single biopsies of the molecular alterations occurring in the various congenital enzyme deficiencies of the human small intestine.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Pharmacology/Neurobiology (Hauri)
UniBasel Contributors:Hauri, Hans-Peter
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:0009-8981
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:20
Deposited On:22 Mar 2012 13:19

Repository Staff Only: item control page