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Quantification of single-stranded nucleic acid and oligonucleotide interactions with metal ions by affinity capillary electrophoresis - Part II

Stettler, Alexandra R. and Chaurin, Valerie and Constable, Edwin C. and Housecroft, Catherine E. and Schwarz, Maria A.. (2008) Quantification of single-stranded nucleic acid and oligonucleotide interactions with metal ions by affinity capillary electrophoresis - Part II. Electrophoresis, 29 (16). pp. 3342-3348.

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Official URL: http://edoc.unibas.ch/dok/A6055995

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Abstract

The interactions between tetranucleotides or heptanucleotides and inorganic cations have been measured by affinity CZE. The variation in migration behavior with increasing concentrations of divalent cations (Ca2+, Mg2+ and Ni2+) in the running buffer was investigated and quantified by the calculation of binding constants for mononuclear and multinuclear interactions. In addition to these fundamental studies of binding equilibria, the effect of sequence and the position of the guanine transition metal-binding site in the oligonucleotide have been investigated.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Former Organization Units Chemistry > Anorganische Chemie (Constable)
05 Faculty of Science > Departement Chemie > Former Organization Units Chemistry > Anorganische Chemie (Housecroft)
UniBasel Contributors:Constable, Edwin Charles and Housecroft, Catherine Elizabeth
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Wiley
ISSN:0173-0835
e-ISSN:1522-2683
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:12 Apr 2017 07:09
Deposited On:01 Feb 2013 08:40

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