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Image Processing of 2D Crystal Images

Arheit, Marcel and Castaño-Díez, Daniel and Thierry, Raphaël and Gipson, Bryant R. and Zeng, Xiangyan and Stahlberg, Henning. (2013) Image Processing of 2D Crystal Images. Methods in Molecular Biology, 955. pp. 171-194.

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Official URL: http://edoc.unibas.ch/dok/A6056066

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Abstract

Electron crystallography of membrane proteins uses cryo-transmission electron microscopy to image frozen-hydrated 2D crystals. The processing of recorded images exploits the periodic arrangement of the structures in the images to extract the amplitudes and phases of diffraction spots in Fourier space. However, image imperfections require a crystal unbending procedure to be applied to the image before evaluation in Fourier space. We here describe the process of 2D crystal image unbending, using the 2dx software system.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Stahlberg)
UniBasel Contributors:Stahlberg, Henning
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Humana Press
ISSN:1064-3745
e-ISSN:1940-6029
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:12 Oct 2017 10:05
Deposited On:04 Jan 2013 08:37

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