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Identification of an African bacillus anthracis lineage that lacks expression of the spore surface associated anthrose containing oligosaccharide

Tamborrini, M. and Bauer, M. and Bolz, M. and Maho, A. and Oberli, M. A. and Werz, D. B. and Schelling, E. and Zinsstag, J. and Seeberger, P. H. and Frey, J. and Pluschke, G.. (2011) Identification of an African bacillus anthracis lineage that lacks expression of the spore surface associated anthrose containing oligosaccharide. Journal of bacteriology : a publication of the American Society for Microbiology, Vol. 193, H. 14. pp. 3506-3511.

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Official URL: http://edoc.unibas.ch/dok/A6002234

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Abstract

The surface of Bacillus anthracis endospores exposes a pentasaccharide containing the monosaccharide anthrose which has been considered as vaccine or target for specific detection of the spores. In this study B. anthracis strains isolated from cattle carcasses in African countries where anthrax is endemic were tested for their cross-reactivity with monoclonal antibodies (mAbs) specific for anthrose containing oligosaccharides. Unexpectedly, none of the isolates collected in Chad, Cameroon and Mali were recognized by the mAbs. Sequencing of the four-gene operon, encoding anthrose biosynthetic enzymes, revealed the presence of premature stop codons in the amino-transferase and the glycosyltransferase genes in all isolates from Chad, Cameroon and Mali. Both immunological and genetic findings suggest that the West African isolates are unable to produce anthrose. The anthrose deficient strains from West Africa belong to a particular genetic lineage. Immunization of cattle in Chad with a locally produced vaccine based on anthrose-positive spores of the B. anthracis strain Sterne elicited an anti-carbohydrate IgG response specific for a synthetic anthrose containing tetrasaccharide as demonstrated by glycan microarray analysis. Competition immunoblots with synthetic pentasaccharide derivatives suggested an immunodominant role of the anthrose containing carbohydrate in cattle. In West Africa anthrax is highly endemic. Massive vaccination of livestock in this area has taken place over long periods of time using spores of the anthrose positive vaccine strain Sterne. Spread of anthrose deficient strains in this region may represent an escape strategy of B. anthracis
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology > Molecular Immunology (Pluschke)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Human and Animal Health > One Health (Zinsstag)
UniBasel Contributors:Pluschke, Gerd and Zinsstag, Jakob Z and Tamborrini, Marco and Bolz, Miriam and Schelling, Esther
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:American Society for Microbiology
ISSN:1098-5530
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:08 Nov 2012 16:23
Deposited On:08 Nov 2012 16:19

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