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YscU recognizes translocators as export substrates of the Yersinia injectisome

Sorg, I. and Wagner, S. and Amstutz, M. and Muller, S. A. and Broz, P. and Lussi, Y. and Engel, A. and Cornelis, G. R.. (2007) YscU recognizes translocators as export substrates of the Yersinia injectisome. The EMBO journal, Vol. 26, H. 12. pp. 3015-3024.

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Official URL: http://edoc.unibas.ch/dok/A5257623

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Abstract

YscU is an essential component of the export apparatus of the Yersinia injectisome. It consists of an N-terminal transmembrane domain and a long cytoplasmic C-terminal domain, which undergoes auto-cleavage at a NPTH site. Substitutions N263A and P264A prevented cleavage of YscU and abolished export of LcrV, YopB and YopD but not of Yop effectors. As a consequence, yscU(N263A) mutant bacteria made needles without the LcrV tip complex and they could not form translocation pores. The graft of the export signal of the effector YopE, at the N-terminus of LcrV, restored LcrV export and assembly of the tip complex. Thus, YscU cleavage is required to acquire the conformation allowing recognition of translocators, which represent an individual category of substrates in the hierarchy of export. In addition, yscU(N263A) mutant bacteria exported reduced amounts of the YscP ruler and made longer needles. Increasing YscP export resulted in needles with normal size, depending on the length of the ruler. Hence, the effect of the yscU(N263A) mutation on needle length was the consequence of a reduced YscP export.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Engel)
05 Faculty of Science > Departement Biozentrum > Infection Biology
05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Molecular Microbiology (Cornelis)
UniBasel Contributors:Broz, Petr and Engel, Andreas H and Cornelis, Guy R. and Sorg, Isabel and Amstutz, Marlise and Müller, Shirley
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Nature Publishing Group
ISSN:0261-4189
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:01 Feb 2013 08:44
Deposited On:22 Mar 2012 13:18

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