Burkavidin: A novel secreted biotin-binding protein from the human pathogen Burkholderia pseudomallei

Sardo, Alessia and Wohlschlager, Therese and Lo, Cheikh and Zoller, Heinz and Ward, Thomas R. and Creus, Marc. (2011) Burkavidin: A novel secreted biotin-binding protein from the human pathogen Burkholderia pseudomallei. Protein Expression and Purification, 77 (2). pp. 131-139.

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Official URL: http://edoc.unibas.ch/dok/A6001509

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The avidin–biotin technology has many applications, including molecular detection; immobilization; protein purification; construction of supramolecular assemblies and artificial metalloenzymes. Here we present the recombinant expression of novel biotin-binding proteins from bacteria and the purification and characterization of a secreted burkavidin from the human pathogen Burkholderia pseudomallei. Expression of the native burkavidin in Escherichia coli led to periplasmic secretion and formation of a biotin-binding, thermostable, tetrameric protein containing an intra-monomeric disulphide bond. Burkavidin showed one main species as measured by isoelectric focusing, with lower isoelectric point (pI) than streptavidin. To exemplify the potential use of burkavidin in biotechnology, an artificial metalloenzyme was generated using this novel protein-scaffold and shown to exhibit enantioselectivity in a rhodium-catalysed hydrogenation reaction.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Bioanorganische Chemie (Ward)
UniBasel Contributors:Creus, Marc and Sardo, Alessia and Lo, Cheikh and Ward, Thomas R.
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:03 May 2017 07:51
Deposited On:11 Oct 2012 15:25

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