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BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons

Dieni, Sandra and Matsumoto, Tomoya and Dekkers, Martijn and Rauskolb, Stefanie and Ionescu, Mihai S. and Deogracias, Ruben and Gundelfinger, Eckart D. and Kojima, Masami and Nestel, Sigrun and Frotscher, Michael and Barde, Yves-Alain. (2012) BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons. The Journal of cell biology, Vol. 196, H. 6. pp. 775-788.

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Official URL: http://edoc.unibas.ch/dok/A6002600

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Abstract

Although brain-derived neurotrophic factor (BDNF) regulates numerous and complex biological processes including memory retention, its extremely low levels in the mature central nervous system have greatly complicated attempts to reliably localize it. Using rigorous specificity controls, we found that antibodies reacting either with BDNF or its pro-peptide both stained large dense core vesicles in excitatory presynaptic terminals of the adult mouse hippocampus. Both moieties were ?10-fold more abundant than pro-BDNF. The lack of postsynaptic localization was confirmed in Bassoon mutants, a seizure-prone mouse line exhibiting markedly elevated levels of BDNF. These findings challenge previous conclusions based on work with cultured neurons, which suggested activity-dependent dendritic synthesis and release of BDNF. They instead provide an ultrastructural basis for an anterograde mode of action of BDNF, contrasting with the long-established retrograde model derived from experiments with nerve growth factor in the peripheral nervous system.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Pharmacology/Neurobiology (Barde)
UniBasel Contributors:Barde, Yves-Alain and Deogracias Pastor, Ruben
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Rockefeller University Press
ISSN:0021-9525
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:31 Dec 2015 10:50
Deposited On:11 Oct 2012 15:18

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