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Automatic recovery of missing amplitudes and phases in tilt-limited electron crystallography of two-dimensional crystals

Gipson, B. R. and Masiel, D. J. and Browning, N. D. and Spence, J. and Mitsuoka, K. and Stahlberg, H.. (2011) Automatic recovery of missing amplitudes and phases in tilt-limited electron crystallography of two-dimensional crystals. Physical review. E, Statistical, nonlinear and soft matter physics, Vol. 84, H. 1, Pt. 1 , 011916.

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Official URL: http://edoc.unibas.ch/dok/A6001517

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Abstract

Electron crystallography of 2D protein crystals provides a powerful tool for the determination of membrane protein structure. In this method, data is acquired in the Fourier domain as randomly sampled, uncoupled, amplitudes and phases. Due to physical constraints on specimen tilting, those Fourier data show a vast un-sampled "missing cone" of information, producing resolution loss in the direction perpendicular to the membrane plane. Based on the flexible language of projection onto sets, we provide a full solution for these problems with a projective constraint optimization algorithm that, for sufficiently oversampled data, produces complete recovery of unmeasured data in the missing cone. We apply this method to an experimental data set of Bacteriorhodopsin and show that, in addition to producing superior results compared to traditional reconstruction methods, full, reproducible, recovery of the missing cone from noisy data is possible. Finally, we present an automatic implementation of the refinement routine as open source, freely distributed, software that will be included in our 2dx software package.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Stahlberg)
UniBasel Contributors:Stahlberg, Henning
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:American Institute of Physicas
ISSN:1063-651X
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:11 Oct 2012 15:31
Deposited On:11 Oct 2012 15:18

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