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Tobacco mutants with reduced microtubule dynamics are less susceptible to TMV

Ouko, M. O. and Sambade, A. and Brandner, K. and Niehl, A. and Pena, E. and Ahad, A. and Heinlein, M. and Nick, P.. (2010) Tobacco mutants with reduced microtubule dynamics are less susceptible to TMV. The plant journal, Vol. 62, H. 5. pp. 829-839.

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Official URL: http://edoc.unibas.ch/dok/A5842322

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Abstract

A panel of seven SR1 tobacco mutants (ATER1 to ATER7) derived via T-DNA activation tagging and screening for resistance to a microtubule assembly inhibitor, ethyl phenyl carbamate, were used to study the role of microtubules during infection and spread of tobacco mosaic virus (TMV). In one of these lines, ATER2, alpha-tubulin is shifted from the tyrosinylated into the detyrosinated form, and the microtubule plus-end marker GFP-EB1 moves significantly slower when expressed in the background of the ATER2 mutant as compared with the SR1 wild type. The efficiency of cell-to-cell movement of TMV encoding GFP-tagged movement protein (MP-GFP) is reduced in ATER2 accompanied by a reduced association of MP-GFP with plasmodesmata. This mutant is also more tolerant to viral infection as compared with the SR1 wild type, implying that reduced microtubule dynamics confer a comparative advantage in face of TMV infection.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Botanik > Pflanzenphysiologie Pathogenabwehr (Boller)
UniBasel Contributors:Heinlein, Manfred
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Blackwell Science
ISSN:0960-7412
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:19 Jul 2013 07:41
Deposited On:11 Oct 2012 15:17

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