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Correlation of membrane / water partition coefficients of detergents with the critical micelle concentration

Heerklotz, H. and Seelig, J.. (2000) Correlation of membrane / water partition coefficients of detergents with the critical micelle concentration. Biophysical journal, Vol. 78, H. 5. pp. 2435-2440.

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Official URL: http://edoc.unibas.ch/dok/A5257409

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Abstract

The membrane/water partition coefficients, K, of 15 electrically neutral (non-charged or zwitterionic) detergents were measured with phospholipid vesicles by using isothermal titration calorimetry, and were compared to the corresponding critical micellar concentrations, cmc. The detergents measured were oligo(ethylene oxide) alkyl ethers (C(m)EO(n) with m = 10/n = 3, 7 and m = 12/n = 3.8); alkylglucosides (octyl, decyl); alkylmaltosides (octyl, decyl, dodecyl); diheptanoylphosphatidylcholine; Tritons (X-100, X-114) and CHAPS. A linear relation between the free energies of partitioning into the membrane and micelle formation was found such that K. CMC approximately 1. The identity K. CMC = 1 was used to classify detergents with respect to their membrane disruption potency. "Strong" detergents are characterized by K. CMC > 1 and solubilize lipid membranes at detergent-to-lipid ratios X(b) > 1 (alkylmaltosides, tritons, heptaethylene glycol alkyl ethers). "Weak" detergents are characterized by K. CMC < 1 and accumulate in the membrane- to detergent-to-lipid ratios X(b) < 1 before the bilayer disintegrates (alkylglucosides, pentaethylene glycol dodecyl ether).
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Biophysical Chemistry (Seelig J)
UniBasel Contributors:Seelig, Joachim
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Biophysical Society
ISSN:0006-3495
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:19
Deposited On:22 Mar 2012 13:18

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