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SOD Antioxidant Nanoreactors : influence of Block Copolymer Composition on the Nanoreactor Efficiency

Onaca, Ozana and Hughes, David W. and Balasubramanian, Vimalkumar and Grzelakowski, Mariusz and Meier, Wolfgang and Palivan, Cornelia G.. (2010) SOD Antioxidant Nanoreactors : influence of Block Copolymer Composition on the Nanoreactor Efficiency. Macromolecular Bioscience, 10 (5). pp. 531-538.

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Official URL: http://edoc.unibas.ch/dok/A5841682

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Abstract

The bioavailability limitations of proteins make them difficult to be directly delivered, particularly in diseases caused by insufficient amts. or inactive variants of those proteins. Nanoreactors represent a new promising approach to overcome these limitations because they serve both to protect the protein in their aq. interior, and simultaneously to allow the protein to act in situ. Here we examine an antioxidant nanoreactor based on SOD encapsulated in amphiphilic block copolymer nanovesicles, and analyze its behavior as a function of the copolymer compn. The membrane of the triblock copolymer nanovesicles plays a double role, both to shield the sensitive protein and selectively to let superoxide and dioxygen penetrate to its inner space. The encapsulation efficiency for different triblock copolymer vesicles was quantified by fluorescence correlation spectroscopy using a fluorescently labeled SOD. Pulse radiolysis expts. and an enzymic assay were used to compare the permeability of the wall-forming membranes towards superoxide anions. While the encapsulation efficiency mainly depends on the vesicle dimensions, the membrane permeability is mainly affected by the length of the hydrophobic PDMS middle blocks of our polymers. For polymers with very long PDMS chains superoxide anion transport across the membranes was too slow to be detected by our expts. [on SciFinder(R)]
Faculties and Departments:05 Faculty of Science > Departement Chemie
05 Faculty of Science > Departement Chemie > Chemie > Makromolekulare Chemie (Meier)
UniBasel Contributors:Meier, Wolfgang P.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Wiley
ISSN:1616-5187
e-ISSN:1616-5195
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:10 Apr 2017 07:43
Deposited On:14 Sep 2012 06:58

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