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Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy

Dalgarno, P. A. and Dalgarno, H. I. C. and Putoud, A. and Lambert, R. and Paterson, L. and Logan, D. C. and Towers, D. P. and Warburton, R. J. and Greenaway, A. H.. (2010) Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy. Optics express, Vol. 18, H. 2. pp. 877-884.

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Official URL: http://edoc.unibas.ch/dok/A5842070

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Abstract

A conventional microscope produces a sharp image from just a single object-plane. This is often a limitation, notably in cell biology. We present a microscope attachment which records sharp images from several object-planes simultaneously. The key concept is to introduce a distorted diffraction grating into the optical system, establishing an order-dependent focussing power in order to generate several images, each arising from a different object-plane. We exploit this multiplane imaging not just for bio-imaging but also for nano-particle tracking, achieving similar to 10 nm z position resolution by parameterising the images with an image sharpness metric. (C)2010 Optical Society of America
Faculties and Departments:05 Faculty of Science > Departement Physik > Physik > Experimental Physics (Warburton)
UniBasel Contributors:Warburton, Richard J
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Optical Society of America
ISSN:1094-4087
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:14 Sep 2012 07:19
Deposited On:14 Sep 2012 06:52

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