Centromere DNA decatenation depends on cohesin removal and is required for mammalian cell division

Wang, L. H. -C. and Mayer, B. and Stemmann, O. and Nigg, E. A.. (2010) Centromere DNA decatenation depends on cohesin removal and is required for mammalian cell division. Journal of cell science, Vol. 123, H. 5. pp. 806-813.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A5842436

Downloads: Statistics Overview


Sister chromatid cohesion is mediated by DNA catenation and proteinaceous cohesin complexes. The recent visualization of PICH (Plk1-interacting checkpoint helicase)-coated DNA threads in anaphase cells raises new questions as to the role of DNA catenation and its regulation in time and space. In the present study we show that persistent DNA catenation induced by inhibition of Topoisomerase-IIalpha can contribute to sister chromatid cohesion in the absence of cohesin complexes and that resolution of catenation is essential for abscission. Furthermore, we use an in vitro chromatid separation assay to investigate the temporal and functional relationship between cohesin removal and Topoisomerase-IIalpha-mediated decatenation. Our data suggest that centromere decatenation can occur only after separase activation and cohesin removal, providing a plausible explanation for the persistence of centromere threads after anaphase onset.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Nigg)
UniBasel Contributors:Nigg, Erich A.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Company of Biologists
Note:Publication type according to Uni Basel Research Database: Journal article
Related URLs:
Identification Number:
Last Modified:08 Jun 2012 06:56
Deposited On:08 Jun 2012 06:51

Repository Staff Only: item control page