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The BatR/BatS two-component regulatory system controls the adaptive response of Bartonella henselae during human endothelial cell infection

Quebatte, Maxime and Dehio, Michaela and Tropel, David and Basler, Andrea and Toller, Isabella and Raddatz, Guenter and Engel, Philipp and Huser, Sonja and Schein, Hermine and Lindroos, Hillevi L. and Andersson, Siv G. E. and Dehio, Christoph. (2010) The BatR/BatS two-component regulatory system controls the adaptive response of Bartonella henselae during human endothelial cell infection. Journal of bacteriology, 192 (13). pp. 3352-3367.

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Official URL: http://edoc.unibas.ch/dok/A5839843

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Abstract

Here, we report the first comprehensive study of Bartonella henselae gene expression during infection of human endothelial cells. Expression of the main cluster of upregulated genes, comprising the VirB type IV secretion system and its secreted protein substrates, is shown to be under the positive control of the transcriptional regulator BatR. We demonstrate binding of BatR to the promoters of the virB operon and a substrate-encoding gene and provide biochemical evidence that BatR and BatS constitute a functional two-component regulatory system. Moreover, in contrast to the acid-inducible (pH 5.5) homologs ChvG/ChvI of Agrobacterium tumefaciens, BatR/BatS are optimally activated at the physiological pH of blood (pH 7.4). By conservation analysis of the BatR regulon, we show that BatR/BatS are uniquely adapted to upregulate a genus-specific virulence regulon during hemotropic infection in mammals. Thus, we propose that BatR/BatS two-component system homologs represent vertically inherited pH sensors that control the expression of horizontally transmitted gene sets critical for the diverse host-associated life styles of the alphaproteobacteria.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Dehio)
UniBasel Contributors:Dehio, Christoph and Qu├ębatte, Maxime and Engel, Philipp
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:American Society for Microbiology
ISSN:1098-5530
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:21 Sep 2017 12:57
Deposited On:08 Jun 2012 06:49

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