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Imaging synaptogenesis by measuring accumulation of synaptic proteins

Dean, Camin and Scheiffele, Peter. (2009) Imaging synaptogenesis by measuring accumulation of synaptic proteins. Cold Spring Harbor Protocols, Vol. 2009, H. 11 , pdb.prot5315.

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Official URL: http://edoc.unibas.ch/dok/A5842495

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Abstract

Synapse formation and modification involve the successive recruitment of pre- and post-synaptic signaling molecules. The procedure presented here investigates the function of specific synaptic factors in gain- and loss-of-function experiments by measuring protein accumulation at synapses using quantitative immunohistochemistry, confocal microscopy, and image analysis. Although the procedure is described for dissociated cultures of hippocampal neurons, it can be extended to various neuronal cell types in culture.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Neurobiology > Cell Biology (Scheiffele)
UniBasel Contributors:Scheiffele, Peter
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:04 Sep 2015 14:31
Deposited On:08 Jun 2012 06:48

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