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MAHRP2, an exported protein of Plasmodium falciparum, is an essential component of Maurer's cleft tethers

Pachlatko, E. and Rusch, S. and Müller, A. and Hemphill, A. and Tilley, L. and Hanssen, E. and Beck, H. P.. (2010) MAHRP2, an exported protein of Plasmodium falciparum, is an essential component of Maurer's cleft tethers. Molecular Microbiology, Vol. 77, H. 5. pp. 1136-1152.

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Official URL: http://edoc.unibas.ch/dok/A5842944

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Abstract

Summary Upon invasion into erythrocytes, the malaria parasite Plasmodium falciparum must refurbish the host cell. The objective of this study was to elucidate the location and function of MAHRP2 in these processes. Using immunofluorescence and immunoelectron microscopy we showed that the Membrane-Associated Histidine-Rich Protein-2 (MAHRP2) is exported during this process to novel cylindrical structures in the erythrocyte cytoplasm. We hypothesize that these structures tether organelles known as Maurer's clefts to the erythrocyte skeleton. Live cell imaging of parasite transfectants expressing MAHRP2-GFP revealed both mobile and fixed populations of the tether-like structures. Differential centrifugation allowed the enrichment of these novel structures. MAHRP2 possesses neither a signal peptide nor a PEXEL motif, and sequences required for export were determined using transfectants expressing truncated MAHRP2 fragments. The first 15 amino acids and the histidine-rich N-terminal region are necessary for correct trafficking of MAHRP2 together with a predicted hydrophobic region. Solubilization studies showed that MAHRP2 is membrane-associated but not membrane spanning. Several attempts to delete the mahrp2 gene failed indicating that the protein is essential for parasite survival
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Former Units within Swiss TPH > Molecular Parasitology and Epidemiology (Beck)
UniBasel Contributors:Beck, Hans-Peter
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Blackwell
ISSN:0950-382X
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:08 Jun 2012 06:55
Deposited On:08 Jun 2012 06:47

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