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Molecular epidemiology of the nasal colonization by methicillin-susceptible staphylococcus aureus in Swiss children

Mégevand, C. and Gervaix, A. and Heininger, U. and Berger, C. and Aebi, C. and Vaudaux, B. and Kind, C. and Gnehm, H.-P. and Hitzler, M. and Renzi, G. and Schrenzel, J. and Fran?ois, P.. (2010) Molecular epidemiology of the nasal colonization by methicillin-susceptible staphylococcus aureus in Swiss children. Clinical microbiology and infection, Vol. 16, no. 9. pp. 1414-1420.

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Official URL: http://edoc.unibas.ch/dok/A5839117

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Abstract

Abstract Nasal carriage of Staphylococcus aureus contributes to an increased risk of developing an infection with the same bacterial strain. Genetic regulatory elements and toxin-expressing genes are virulence factors associated with the pathogenic potential of S. aureus. Our study aimed at performing an extensive molecular characterization of methicillin-susceptible S. aureus (MSSA) carried by children. MSSA were recovered from the nostrils of children. Panton-Valentine leukocidin (PVL), exfoliatins A and B (exfoA and exfoB), as well as the toxic-shock staphylococcal toxin (TSST-1) and agr group typing were determined by qPCR. Multiple-locus variable-number of tandem repeat analysis (MLVA) assay was also performed for genotyping. Five hundred seventy two strains of MSSA were analyzed. Overall, 30.1% of S. aureus were positive for toxin-expressing genes. 28.5% contained one toxin and 1.6% two toxins. The most commonly detected toxin gene was tst, present in 145 (25,4%) strains. TSST-1 gene was significantly associated with the agr group 3 (OR=56.8, 95%CI=32.0-100.8). MLVA analysis revealed a large diversity of genetic content and no clonal relationship was demonstrated between analyzed MSSA strains. MLST confirmed this observation of diversity and identified ST45 as a frequent colonizer. Molecular characterization of MSSA revealed an extraordinary diversity in terms of genomic content and important virulence factors. This broad diversity in MSSA carriage strains suggests a limited selection pressure in our geographic area.
Faculties and Departments:03 Faculty of Medicine > Bereich Kinder- und Jugendheilkunde (Klinik) > Kinder- und Jugendheilkunde (UKBB) > Infektiologie und Vakzinologie (Heininger/Rudin)
03 Faculty of Medicine > Departement Klinische Forschung > Bereich Kinder- und Jugendheilkunde (Klinik) > Kinder- und Jugendheilkunde (UKBB) > Infektiologie und Vakzinologie (Heininger/Rudin)
03 Faculty of Medicine > Bereich Kinder- und Jugendheilkunde (Klinik) > Kinder- und Jugendheilkunde (UKBB)
03 Faculty of Medicine > Departement Klinische Forschung > Bereich Kinder- und Jugendheilkunde (Klinik) > Kinder- und Jugendheilkunde (UKBB)
UniBasel Contributors:Heininger, Ulrich
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Blackwell
ISSN:1198-743X
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:05 Dec 2014 09:45
Deposited On:08 Jun 2012 06:30

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