edoc

Vaccinated students with negative enzyme immunoassay results show positive measles virus-specific antibody levels by immunofluorescence and plaque neutralisation tests

Tischer, Annedore and Gassner, Markus and Richard, Jean-Luc and Suter-Riniker, Franziska and Mankertz, Annette and Heininger, Ulrich. (2007) Vaccinated students with negative enzyme immunoassay results show positive measles virus-specific antibody levels by immunofluorescence and plaque neutralisation tests. Journal of clinical virology, Vol. 38, H. 3. pp. 204-209.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A5839126

Downloads: Statistics Overview

Abstract

BACKGROUND: The prevalence of measles antibodies was investigated by an enzyme immunoassay (EIA) in students aged 14 every year since 1996 in a Swiss municipality. This region has wide measles vaccine coverage (first dose < or = 95%, second dose < or = 65%) without any reported measles outbreaks since 20 years. In 2003 and 2004, in contrast to previous years, surprisingly many negative results (33% and 54%, respectively) were observed. OBJECTIVES: To corroborate the measles antibody values by different methods. STUDY DESIGN: Serum samples from 101 students with known vaccination status were available. Sera with equivocal and negative results obtained by two different EIAs were retested by indirect immunofluorescence test (IFT) and plaque neutralisation test (PNT). RESULTS: Retesting by IFT showed a positive result in 17/21 sera (81%) and retesting by PNT indicated that 46/49 sera (94%) were positive; the three sera with negative PNT result were from unvaccinated individuals. Only 3/96 vaccinated students showed measles antibodies below the putative protective level of 0.2 IU/ml after retesting by PNT. CONCLUSIONS: Negative EIA results should be interpreted with caution in a widely vaccinated population without booster by circulation of wild viruses. Retesting by IFT or PNT is recommended.
Faculties and Departments:03 Faculty of Medicine > Bereich Kinder- und Jugendheilkunde (Klinik) > Kinder- und Jugendheilkunde (UKBB)
03 Faculty of Medicine > Departement Klinische Forschung > Bereich Kinder- und Jugendheilkunde (Klinik) > Kinder- und Jugendheilkunde (UKBB)
03 Faculty of Medicine
UniBasel Contributors:Heininger, Ulrich
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:1386-6532
Note:Publication type according to Uni Basel Research Database: Journal article
Related URLs:
Identification Number:
Last Modified:08 Jun 2012 06:53
Deposited On:08 Jun 2012 06:30

Repository Staff Only: item control page